Novel expression and function of peroxisome proliferator-activated receptor gamma (PPARgamma) in human neuroblastoma cells.

Abstract

Peroxisome proliferator-activated receptor gamma (PPAR-gamma) is a member of the nuclear receptor superfamily of ligand-dependent transcription factors that has been shown to play a major role in adipocyte and monocyte/macrophage differentiation. Recent work has also suggested a role for PPARgamma in cell cycle control and/or differentiation of other cell types including breast and lung cancer cells. Using reverse transcription-PCR, we now show for the first time that human neuroblastoma (nb) cells express PPARbeta and -gamma, but not -alpha. Using the LA-N-5 nb cell line, we have determined that the natural PPARgamma ligand 15-deoxy-delta prostaglandin J2, as well as the synthetic PPARgamma agonist GW1929, can stimulate the differentiation of nb cells, as evidenced by the inhibition of cell proliferation, neurite outgrowth, increased acetylcholinesterase activity, and the reduction of N-myc expression. We have also demonstrated that PPARgamma is expressed in primary nb and, furthermore, that the expression of this receptor correlates with the maturational stage of the nb cells. Taken together, these studies have implicated a role for PPARgamma in peripheral nerve cell biology and suggest that the PPARgamma signaling pathway is involved in the regulation of nb cell growth and differentiation.

• Authors: S W Han, M E Greene, J Pitts, R K Wada, N Sidell
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 7 Issue 1 Pg. 98-104 (Jan 2001) ISSN: 1078-0432 [Print] United States
• PMID: 11205925 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA Primers
• RNA, Messenger
• Receptors, Cytoplasmic and Nuclear
• Transcription Factors
• Acetylcholinesterase

Topics

• Acetylcholinesterase (analysis)
• Blotting, Northern
• Cell Differentiation
• DNA Primers (chemistry)
• Genes, myc (genetics, physiology)
• Humans
• Immunoenzyme Techniques
• Neuroblastoma (metabolism, pathology)
• RNA, Messenger (metabolism)
• Receptors, Cytoplasmic and Nuclear (biosynthesis, genetics)
• Reverse Transcriptase Polymerase Chain Reaction
• Transcription Factors (biosynthesis, genetics)
• Tumor Cells, Cultured (drug effects, metabolism)