NBP is the p53 homolog p63.

Abstract	We previously identified a non-p53, p53-responsive DNA element (p53RE)-binding protein named NBP, functionally analogous to p53, from human cervical carcinoma Hela cells. Here we report a biochemical study demonstrating that this activity is the recently cloned p53 analog p63. NBP was purified through conventional and DNA affinity chromatography to apparent homogeneity with a prominent polypeptide migrating in between the 43 and 68 kDa positions on a SDS gel. This polypeptide immunoreacted with monoclonal anti-p63 but not anti-p53 or anti-p73 antibodies. Also, NBP co-purified with p63 through each step of fractionation, as detected with anti-p63 antibodies. DNA-protein complexes formed with purified NBP and p53RE-containing oligomers derived from the p21(waf1) promoter were supershifted by anti-p63 but not anti-p53 antibodies. Thus, these results demonstrate that NBP is encoded by the p53 homolog p63 gene.
Authors	X Zeng, Y Zhu, H Lu
Journal	Carcinogenesis (Carcinogenesis) Vol. 22 Issue 2 Pg. 215-9 (Feb 2001) ISSN: 0143-3334 [Print] England
PMID	11181441 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References	CKAP4 protein, human DNA-Binding Proteins Membrane Proteins Nuclear Proteins Phosphoproteins Son protein, mouse TP63 protein, human Trans-Activators Transcription Factors Trp63 protein, mouse Tumor Suppressor Protein p53 Tumor Suppressor Proteins
Topics	Blotting, Western DNA-Binding Proteins (chemistry, isolation & purification, metabolism) Electrophoresis, Agar Gel Electrophoresis, Polyacrylamide Gel Female Genes, Tumor Suppressor HeLa Cells (chemistry) Humans Immunoblotting Membrane Proteins Nuclear Proteins (chemistry, isolation & purification, metabolism) Ovarian Neoplasms (pathology) Phosphoproteins (chemistry, metabolism) Trans-Activators Transcription Factors Tumor Suppressor Protein p53 (chemistry, metabolism) Tumor Suppressor Proteins Uterine Cervical Neoplasms (pathology)

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