Abstract |
Vesicular stomatitis virus matrix protein (VSV M) has been shown to inhibit both transcription and nucleocytoplasmic transport. We have isolated a mutant form of M, termed M(D), lacking both inhibitory activities. HeLa cells expressing M, but not M(D), accumulate polyadenylated RNAs within the nucleus. Concomitantly, a fraction of M, but not of the M(D) mutant, localizes at the nuclear rim. Additionally, the nucleoporin Nup98 specifically interacts with M but not with M(D). In Nup98(-/-) cells, both the levels of M at the nuclear envelope and its inhibitory effects on host cell-directed expression of reporter genes were significantly reduced. Together, our data demonstrate that VSV M inhibits host cell gene expression by targeting a nucleoporin and primarily blocking nuclear export.
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Authors | von Kobbe C, van Deursen JM, J P Rodrigues, D Sitterlin, A Bachi, X Wu, M Wilm, M Carmo-Fonseca, E Izaurralde |
Journal | Molecular cell
(Mol Cell)
Vol. 6
Issue 5
Pg. 1243-52
(Nov 2000)
ISSN: 1097-2765 [Print] United States |
PMID | 11106761
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- M protein, Vesicular stomatitis virus
- Nuclear Pore Complex Proteins
- RNA, Messenger
- Viral Matrix Proteins
- nuclear pore complex protein 98
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Topics |
- Active Transport, Cell Nucleus
- Amino Acid Sequence
- Animals
- Cell Line
- Cell Nucleus
(genetics, metabolism)
- Gene Expression Regulation
- HeLa Cells
- Humans
- Mice
- Mice, Knockout
- Molecular Sequence Data
- Mutation
(genetics)
- Nuclear Pore
(chemistry, metabolism)
- Nuclear Pore Complex Proteins
(chemistry, deficiency, genetics, metabolism)
- Protein Binding
- Protein Structure, Tertiary
- RNA, Messenger
(genetics, metabolism)
- Repetitive Sequences, Amino Acid
- Substrate Specificity
- Transcription, Genetic
- Viral Matrix Proteins
(chemistry, genetics, metabolism)
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