Inhibitory effects of synthetic beta peptide on invasion and metastasis of liver cancer.

Abstract

PURPOSE:

To study the inhibitory effects of synthetic beta peptide on invasion and metastasis of liver cancer.

METHODS:

Membrane-type intercellular adhesion molecule-1 (ICAM-1) expression of SMMC-7721 cultured hepatoma cells (7721 cells) was detected by immunofluorescence cell flowmeter. The adhesion of 7721 cells to fibronectin (FN) was assayed by the MTT method. The adhesion of 7721 cells to 7721 cells, 7721 cells to endothelial cells, and 7721 cells to lymphocyte cells was detected by adhesion assay. LCI-D20 human liver cancer metastasis model in nude mice was used in this experiment. One hundred micrograms of beta peptide per mouse were injected subcutaneously after tumor was resected premetastatically or postmetastatically to observe its effect on liver cancer metastasis after hepatectomy.

RESULTS:

Membrane-type ICAM-1 expression of SMMC-7721 cells treated by beta peptide was lower than that of the untreated cells. The adhesion of 7721 cells to FN, 7721 cells to 7721 cells, 7721 cells to endothelial cells, and 7721 cells to lymphocyte cells was also lower in the beta peptide group than in the untreated group.

CONCLUSIONS:

beta Peptide can block the adhesion of 7721 cells to FN, 7721 cells to some host cells in vitro, and inhibit HCC metastasis of LCI-D20 model posthepatectomy in vivo, so it could potentially act as an antimetastasis drug.

• Authors: J J Sun, X D Zhou, Y K Liu, Z Y Tang, R X Sun, Y Zhao, T Uemura
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 126 Issue 10 Pg. 595-600 (Oct 2000) ISSN: 0171-5216 [Print] Germany
• PMID: 11043397 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• CD18 Antigens
• Intercellular Adhesion Molecule-1

Topics

• Animals
• Antineoplastic Agents (chemical synthesis, pharmacology)
• CD18 Antigens (pharmacology)
• Carcinoma, Hepatocellular (metabolism, prevention & control, secondary)
• Cell Adhesion
• Endothelium, Vascular
• Flow Cytometry
• Gene Expression Regulation, Neoplastic
• Humans
• Intercellular Adhesion Molecule-1 (metabolism)
• Liver Neoplasms (metabolism, pathology)
• Lymphocytes
• Mice
• Mice, Nude
• Neoplasm Invasiveness
• Tumor Cells, Cultured
• Umbilical Veins