A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis using a purified fraction from Taenia solium cysticerci.

Abstract	A quantitative enzyme-linked immunosorbent assay (ELISA) for the immunodiagnosis of neurocysticercosis is described. The ELISA was standardized using a purified Taenia solium cysticerci fraction (PCF) obtained by ion exchange chromatography. The ELISA using PCF (PCF-ELISA) and a qualitative ELISA using a whole extract from T. solium cysticerci (WEC-ELISA) were used to screen for cysticercus-specific IgG antibodies in cerebrospinal fluid (CSF) samples from 57 patients with neurocysticercosis and 50 patients with other neurologic disorders. The sensitivity of both assays was 95%, whereas the specificities of PCF-ELISA and WEC-ELISA were 100% and 92%, respectively. The excellent sensitivity and specificity of the PCF-ELISA make this assay a potentially useful tool in screening for antibodies against T. solium cysticerci.
Authors	A D da Silva, E M Quagliato, C L Rossi
Journal	Diagnostic microbiology and infectious disease (Diagn Microbiol Infect Dis) Vol. 37 Issue 2 Pg. 87-92 (Jun 2000) ISSN: 0732-8893 [Print] United States
PMID	10863102 (Publication Type: Journal Article)
Chemical References	Antibodies, Helminth Antigens, Helminth
Topics	Animals Antibodies, Helminth (blood, cerebrospinal fluid) Antigens, Helminth (immunology) Chromatography, Ion Exchange Cysticercus (immunology) Enzyme-Linked Immunosorbent Assay (methods) Humans Neurocysticercosis (diagnosis) Sensitivity and Specificity

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