Glutathione S-transferase-pi gene expression and platinum drug exposure in human lung cancer.

Abstract

We examined the association between the gene expression levels of glutathione S-transferase-pi (GST-pi) and platinum drug exposure in human lung cancer. First we monitored GST-pi gene expression levels in two lung cancer cell lines and in peripheral mononuclear cells of ten previously untreated lung cancer patients after platinum drug exposure. Next we examined GST-pi gene expression levels in 40 lung cancer autopsy specimens. The GST-pi gene expression levels were assessed by the quantitative reverse transcription-polymerase chain reaction or Northern blot analysis. The GST-pi gene expression was not induced by platinum drugs either in vitro and in vivo within 24 h of exposure. In contrast, GST-pi gene expression levels in lung cancer tissues of patients who had been exposed to platinum drugs at least 1 month before death were significantly higher than that in those of patients who had not been exposed. These results suggest that GST-pi gene expression is associated with chronic exposure to platinum drugs in lung cancer and/or the stress response to xenobiotics.

• Authors: T Oguri, Y Fujiwara, O Katoh, H Daga, N Ishikawa, K Fujitaka, M Yamasaki, M Yokozaki, T Isobe, S Ishioka, M Yamakido
• Journal: Cancer letters (Cancer Lett) Vol. 156 Issue 1 Pg. 93-9 (Aug 01 2000) ISSN: 0304-3835 [Print] Ireland
• PMID: 10840164 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Isoenzymes
• Organoplatinum Compounds
• Transcription Factor AP-1
• GSTP1 protein, human
• Glutathione S-Transferase pi
• Glutathione Transferase

Topics

• Antineoplastic Agents (pharmacology)
• Gene Expression Regulation, Enzymologic
• Glutathione S-Transferase pi
• Glutathione Transferase (genetics, physiology)
• Humans
• Isoenzymes (genetics, physiology)
• Lung Neoplasms (drug therapy, enzymology, genetics)
• Organoplatinum Compounds (metabolism, pharmacology)
• Transcription Factor AP-1 (physiology)
• Tumor Cells, Cultured