Abstract |
The growth and spread of tumour cells depends on adequate vasculature. We have previously reported that the expression of interleukin-8 (IL-8) directly correlates with the vascularity of human gastric carcinomas. To provide evidence for a causal role of IL-8 in angiogenesis and tumorigenicity of human gastric cancer, we used the lipofectin method to stably transfect the human TMK-1 gastric carcinoma cells (low endogenous IL-8) with an IL-8 expression vector or control vector. Transfection with IL-8 did not affect the proliferation of cultured cells, yet the culture supernatants of the transfected (but not control) cells stimulated proliferation of human umbilical vein endothelial cells. The IL-8-transfected and control cells were injected into the gastric wall of nude mice. IL-8-transfected cells produced rapidly growing, highly vascular neoplasms as compared to control cells. These results provide direct evidence for the role of IL-8 in the angiogenesis and tumorigenicity of human gastric carcinomas.
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Authors | Y Kitadai, Y Takahashi, K Haruma, K Naka, K Sumii, H Yokozaki, W Yasui, N Mukaida, Y Ohmoto, G Kajiyama, I J Fidler, E Tahara |
Journal | British journal of cancer
(Br J Cancer)
Vol. 81
Issue 4
Pg. 647-53
(Oct 1999)
ISSN: 0007-0920 [Print] England |
PMID | 10574250
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Animals
- Humans
- Interleukin-8
(genetics, physiology)
- Mice
- Mice, Nude
- Neoplasm Transplantation
- Neovascularization, Pathologic
(etiology)
- Stomach Neoplasms
(blood supply, etiology, pathology)
- Transfection
- Transplantation, Heterologous
- Tumor Cells, Cultured
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