We investigated the effects of selective and non-selective
endothelin (ET) antagonists on
warm ischemia-
reperfusion injury of the early phase in the murine liver under
propofol anesthesia. We examined portal pressures, O2 consumptions and liver
enzymes to estimate
injuries using perfused isolated murine liver model. Experiment 1: we compared the perfusate only group, the soy oil (as vehicle) group, and the
propofol group without ischemic loading to determine whether
propofol and its vehicles themselves have any effect. Experiment 2: we determined the effects of ET antagonists on
reperfusion injury after
warm ischemia of 40 minute's cutoff of perfusate under
propofol anesthesia and compared five groups up to 90 min after reperfusion; the
propofol only group (control; 4 mg.l-1), the
BQ-485 group (20 microM), the
BQ-788 group (50 microM), the
bosentan group (40 microM), and the
BQ-485 +
BQ-788 group. The soy oil group showed significantly more liver damage, and the
propofol group showed no damage. In addition,
propofol did not alter the effect of ET antagonists on
reperfusion injury. Clinical dose of
propofol did not seem to alter the effects of ET antagonists on the murine liver in the early phase of
ischemia-reperfusion injury.