Apolipoproteins J and E co-localise with amyloid in gelatinous drop-like and lattice type I corneal dystrophies.

Abstract

AIMS:

Apolipoprotein J (apoJ) and apolipoprotein E (apoE) are thought to contribute to amyloid formation in patients with Alzheimer's disease. The aim of this investigation was to discover whether or not these apolipoproteins associate with corneal amyloid in gelatinous drop-like corneal dystrophy (GDCD) and lattice corneal dystrophy type I (LCD-I).

METHODS:

Corneas from three eyes of three patients with GDCD and one eye of one patient with LCD-I were examined immunohistochemically using antibodies against apoJ and apoE. Two normal corneas were similarly examined. Tissue sections of brain from a patient with Alzheimer's disease were used as positive controls for the antibodies. For all negative controls, mouse IgG was used instead of the primary antibody.

RESULTS:

Intense apoJ and apoE immunoreactivities were found in congophilic amyloid deposits in GDCD and LCD-I. These deposits were located subepithelially in GDCD, and subepithelially and intrastromally in LCD-I. In GDCD, immunostaining of subepithelial amyloid with anti-apoJ was noticeably stronger than with anti-apoE.

CONCLUSIONS:

As in senile plaques in brain from a patient with Alzheimer's disease, apoJ and apoE co-localise with amyloid in corneas with GDCD and LCD-I.

• Authors: K Nishida, A J Quantock, A Dota, N H Choi-Miura, S Kinoshita
• Journal: The British journal of ophthalmology (Br J Ophthalmol) Vol. 83 Issue 10 Pg. 1178-82 (Oct 1999) ISSN: 0007-1161 [Print] England
• PMID: 10502582 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amyloid
• Apolipoproteins
• Apolipoproteins E
• CLU protein, human
• Clusterin
• Complement Inactivator Proteins
• Glycoproteins
• Molecular Chaperones

Topics

• Aged
• Aged, 80 and over
• Alzheimer Disease (metabolism)
• Amyloid (metabolism)
• Apolipoproteins (metabolism)
• Apolipoproteins E (metabolism)
• Clusterin
• Complement Inactivator Proteins (metabolism)
• Corneal Dystrophies, Hereditary (metabolism)
• Female
• Glycoproteins (metabolism)
• Humans
• Male
• Middle Aged
• Molecular Chaperones