Abstract |
The objective of the present study was to clarify the differential effects of endothelinA (ETA) and ETB antagonism in the early phase of ischemia- reperfusion damage. Male Sprague Dawley rats were randomly divided into 4 groups: control (n = 10), bosentan (40 nM; n = 10), BQ-485 (20 nM; n = 10), and BQ-788 (50 nM; n = 10) to compare the effects of ETA or ETB or both ETA and ETB antagonists against the warm ischemia- reperfusion damage of murine livers. Isolated livers were perfused with oxygenated Krebs-Henseleit bicarbonate buffer solution and ET antagonists for 30 min before inducing warm ischemia (non-recirculating system). After 40 min without perfusate, measurements (portal pressure, O2 tensions of influent and effluent perfusate, liver enzymes, etc.) were taken up to 60 min after reperfusion. The BQ-788 group had significantly more liver damage than did the other groups, and more O2 consumption than did the bosentan group. BQ-485 and bosentan were more protective at some points after reperfusion. Antagonism of only the ETB receptors is detrimental, but antagonism of the ETA receptors appears to have a role in protecting the liver from warm ischemia- reperfusion damage in the early phase.
|
Authors | K Tanaka, H Higuchi, F Karasawa, T Sato |
Journal | Research communications in molecular pathology and pharmacology
(Res Commun Mol Pathol Pharmacol)
Vol. 103
Issue 2
Pg. 129-38
(Feb 1999)
ISSN: 1078-0297 [Print] United States |
PMID | 10461680
(Publication Type: Journal Article)
|
Chemical References |
- Azepines
- Endothelin Receptor Antagonists
- Oligopeptides
- Piperidines
- Sulfonamides
- BQ 485
- BQ 788
- L-Lactate Dehydrogenase
- Aspartate Aminotransferases
- Alanine Transaminase
- Bosentan
|
Topics |
- Alanine Transaminase
(pharmacology)
- Animals
- Aspartate Aminotransferases
(metabolism)
- Azepines
(pharmacology, therapeutic use)
- Bosentan
- Endothelin Receptor Antagonists
- L-Lactate Dehydrogenase
(pharmacology)
- Liver
(drug effects, enzymology, injuries)
- Male
- Mice
- Oligopeptides
(pharmacology, therapeutic use)
- Oxygen Consumption
- Piperidines
(pharmacology, therapeutic use)
- Portal Pressure
(drug effects)
- Rats
- Rats, Sprague-Dawley
- Reperfusion Injury
(prevention & control)
- Sulfonamides
(pharmacology, therapeutic use)
|