Molecular cloning and characterization of the human NUDC gene.

Abstract	In both Aspergillus nidulans and the mouse, studies of the nuclear distribution gene C (NudC) have strongly suggested that the NudC protein interacts with NudF, which is the product of NudF, a homologue of human LIS1 (also know as PAFAH1B1), one of the causative genes for classical lissencephaly. We have isolated the human NUDC gene and its two processed pseudogenes. The human NUDC gene is highly conserved and its predicted amino acid sequence shows 94% identity to mouse NudC and 95% identity to rat NudC. The genomic structure of NUDC, its chromosomal localization, and expression pattern in human tissues were characterized. NUDC consists of at least 9 exons ranging from 66 bp to 266 bp in size and 8 introns from 92 bp to 2.0 kb in length, and the total genomic region spans about 8 kb. NUDC was mapped to 1p34-p35 by fluorescence in situ hybridization. Northern analysis showed a major 1.6 kb transcript in all fetal and adult tissues examined. Primers which amplify individual exons of NUDC were developed for mutation analysis.
Authors	N Matsumoto, D H Ledbetter
Journal	Human genetics (Hum Genet) Vol. 104 Issue 6 Pg. 498-504 (Jun 1999) ISSN: 0340-6717 [Print] Germany
PMID	10453739 (Publication Type: Journal Article)
Chemical References	Cell Cycle Proteins Microtubule-Associated Proteins NUDC protein, human Nuclear Proteins Nudc protein, mouse Proteins 1-Alkyl-2-acetylglycerophosphocholine Esterase PAFAH1B1 protein, human Pafah1b1 protein, mouse
Topics	1-Alkyl-2-acetylglycerophosphocholine Esterase Amino Acid Sequence Animals Base Sequence Brain (metabolism) Cell Cycle Proteins Chromosome Mapping Chromosomes, Human, Pair 1 Chromosomes, Human, Pair 2 Cloning, Molecular Exons Humans In Situ Hybridization, Fluorescence Introns Mice Microtubule-Associated Proteins Models, Genetic Molecular Sequence Data Nuclear Proteins Proteins (genetics, metabolism) Rats Sequence Homology, Amino Acid Tissue Distribution

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