Abstract | OBJECTIVE: The aim of this study was to evaluate the possible role of prostacyclin (PGI2) in the pathogenesis of hypertension in spontaneously hypertensive rats (SHR). METHODS: Measurement of mRNA and protein levels of PGH synthase (PGHS)-1, PGI2 synthase and the PGI2 receptor, in the thoracic aorta was performed in SHR aged 5, 10, 20, and 40 weeks old and in age-matched normotensive Wistar-Kyoto (WKY) rats with a competitive polymerase chain reaction method and immunoblotting. Aortic production of 6-keto-PGF1 alpha, the main metabolite of PGI2, was also measured. RESULTS: Compared with age-matched WKY rats, PGHS-1 mRNA and protein levels in the thoracic aorta of SHR increased with age, reaching three- and twofold higher than WKY rats at 40 weeks old, respectively. PGI2 synthase mRNA and protein levels in SHR were significantly higher than in WKY rats at 20 and 40 weeks old. In contrast, PGI2 receptor mRNA levels in SHR were consistently lower than in WKY rats at all ages. CONCLUSIONS:
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Authors | Y Numaguchi, M Harada, H Osanai, K Hayashi, Y Toki, K Okumura, T Ito, T Hayakawa |
Journal | Cardiovascular research
(Cardiovasc Res)
Vol. 41
Issue 3
Pg. 682-8
(Mar 1999)
ISSN: 0008-6363 [Print] England |
PMID | 10435040
(Publication Type: Journal Article)
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Chemical References |
- RNA, Messenger
- Receptors, Epoprostenol
- Receptors, Prostaglandin
- Cytochrome P-450 Enzyme System
- Prostaglandin-Endoperoxide Synthases
- Intramolecular Oxidoreductases
- prostacyclin synthetase
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Topics |
- Aging
(metabolism)
- Animals
- Aorta, Thoracic
(metabolism)
- Cytochrome P-450 Enzyme System
(analysis, genetics)
- Gene Expression
- Hypertension
(metabolism)
- Intramolecular Oxidoreductases
(analysis, genetics)
- Male
- Polymerase Chain Reaction
- Prostaglandin-Endoperoxide Synthases
(genetics, isolation & purification)
- RNA, Messenger
(analysis)
- Rats
- Rats, Inbred SHR
- Rats, Inbred WKY
- Receptors, Epoprostenol
- Receptors, Prostaglandin
(genetics)
- Reverse Transcriptase Polymerase Chain Reaction
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