Abstract |
The molecular basis of T-cell-mediated recognition of ovarian cancer cells remains to be fully addressed. In this study we investigated HLA class I restriction and directed antigens of cytotoxic T lymphocytes (CTL) at the sites of ovarian cancer. Three HLA-class-I-restricted CTL lines were established from the tumor sites of ovarian cancer by culturing tumor-infiltrating lymphocytes or tumor-associated ascitic lymphocytes with interleukin-2: (1) HLA-A2402-restricted and ovarian- adenocarcinoma-specific CTL, (2) HLA-A2-restricted CTL recognizing histologically different cancers, and (3) HLA-B52-restricted and ovarian-cancer-specific CTL. HLA-A0201, HLA-A0206 and HLA-A0207 tumor cells were lysed by the HLA-A2-restricted CTL. HLA-B52 restriction of the third CTL line was confirmed by the transfection of HLA-B5201 cDNA into the tumor cells. The HLA-A2-restricted CTL recognized the SART-1, but not the MAGE-1 or MAGE-3 antigen. These results may facilitate a better understanding of the molecular basis of tumor-specific immunity at the tumor site of ovarian cancer.
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Authors | A Yamada, K Kawano, N Harashima, F Niiya, K Nagai, T Kobayashi, T Mine, K Ushijima, T Nishida, K Itoh |
Journal | Cancer immunology, immunotherapy : CII
(Cancer Immunol Immunother)
1999 May-Jun
Vol. 48
Issue 2-3
Pg. 147-52
ISSN: 0340-7004 [Print] Germany |
PMID | 10414469
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- Histocompatibility Antigens Class I
- MAGEA1 protein, human
- MAGEA3 protein, human
- Melanoma-Specific Antigens
- Neoplasm Proteins
- Ribonucleoproteins, Small Nuclear
- SART1 protein, human
- Interferon-gamma
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Topics |
- Animals
- Antigens, Neoplasm
(immunology)
- COS Cells
- Female
- Histocompatibility Antigens Class I
(physiology)
- Humans
- Interferon-gamma
(biosynthesis)
- Melanoma-Specific Antigens
- Neoplasm Proteins
(immunology)
- Ovarian Neoplasms
(immunology)
- Ribonucleoproteins, Small Nuclear
- T-Lymphocytes, Cytotoxic
(immunology)
- Tumor Cells, Cultured
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