Construction of a recombinant herpesvirus expressing the jellyfish green fluorescent protein.

Abstract

Here we report the insertion of a synthetic version of the cDNA encoding the jellyfish (Aequorea victoria) green fluorescent protein (gfph ) into the genome of pseudorabies (Aujeszky's disease) virus (PrV). A putative latency promoter (PLAT) located at the inverted repeat region of the PrV genome was chosen as the target site for the insertion. Recombinant viral DNA designated as vLAT-gfp was generated as a result of homologous recombination between the transfected viral DNA and a plasmid containing the GFP-expression cassette flanked by viral sequences homologous to the target region. Plaques containing recombinant virus were selected visually using a fluorescent microscope. We demonstrated a GFP-expression in infected neurons of rat brain which showed normal morphology at early stage of viral infection by monitoring fluorescent light emission.

• Authors: Z Boldogköi, F Erdélyi, A Sik, T F Freund, I Fodor
• Journal: Luminescence : the journal of biological and chemical luminescence (Luminescence) 1999 Mar-Apr Vol. 14 Issue 2 Pg. 69-74 ISSN: 1522-7235 [Print] England
• PMID: 10398563 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 1999 John Wiley & Sons, Ltd.

Chemical References

• DNA, Complementary
• Luminescent Proteins
• Recombinant Proteins
• Green Fluorescent Proteins

Topics

• Animals
• Brain (cytology)
• Cell Line
• DNA, Complementary
• Gene Transfer Techniques
• Green Fluorescent Proteins
• Herpesvirus 1, Suid (genetics, isolation & purification)
• Luminescent Proteins (analysis, biosynthesis, genetics)
• Mice
• Neurons (cytology)
• Promoter Regions, Genetic
• Rats
• Recombinant Proteins (analysis, biosynthesis)
• Recombination, Genetic
• Scyphozoa
• Swine
• Tumor Cells, Cultured