Abstract |
The mechanisms underlying the impaired immune response frequently observed in cancer patients are not fully understood. Alteration of T-cell-associated signal transduction molecules has recently been implicated in immune suppression in tumor-bearing hosts. Furthermore, T cells from tumor-bearing host, irrespective of the presence of the zeta-chain, showed a lack of proliferative activity and cytotoxic function. In the present study, we investigated the expression of the zeta-chain molecule and the p56(lck) and p59(fyn) protein tyrosine kinase (PTK) levels in peripheral blood T lymphocytes (T-PBL) from patients with advanced gastric carcinomas; for this, flow cytometric analysis and immunoblotting, respectively, were used. We also compared the results of flow cytometric analysis of PBL between stomach cancer patients and normal healthy volunteers. In T-PBL from 22 tumor-bearing hosts, significantly reduced zeta-chain expression (16/22, 73%) was observed. Moreover, the expression level of p56(lck) in T-PBL from patients was significantly lower than that of p59(fyn). Flow cytometric analysis of T-PBL indicated a markedly increased CD8+28- cell population in T-PBL from 19 tumor-bearing hosts.
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Authors | C W Kim, S H Choi, E J Chung, M J Lee, E K Byun, M H Ryu, Y J Bang |
Journal | Pathobiology : journal of immunopathology, molecular and cellular biology
(Pathobiology)
1999 May-Jun
Vol. 67
Issue 3
Pg. 123-8
ISSN: 1015-2008 [Print] Switzerland |
PMID | 10394132
(Publication Type: Journal Article)
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Chemical References |
- CD28 Antigens
- CD3 Complex
- Membrane Proteins
- Proto-Oncogene Proteins
- Receptor-CD3 Complex, Antigen, T-Cell
- Receptors, Antigen, T-Cell
- antigen T cell receptor, zeta chain
- FYN protein, human
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
- Proto-Oncogene Proteins c-fyn
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Topics |
- Adult
- Aged
- CD28 Antigens
(metabolism)
- CD3 Complex
(metabolism)
- Female
- Flow Cytometry
- Humans
- Immunophenotyping
- Lymphocyte Count
- Lymphocyte Specific Protein Tyrosine Kinase p56(lck)
(metabolism)
- Lymphocytes
(cytology, immunology)
- Male
- Membrane Proteins
(metabolism)
- Middle Aged
- Proto-Oncogene Proteins
(metabolism)
- Proto-Oncogene Proteins c-fyn
- Receptor-CD3 Complex, Antigen, T-Cell
(metabolism)
- Receptors, Antigen, T-Cell
(metabolism)
- Signal Transduction
(immunology)
- Stomach Neoplasms
(blood, immunology)
- T-Lymphocytes
(cytology, immunology)
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