Abstract |
Chinese hamster primary embryonic cells (at G1 phase) were treated with 1.0 or 3.0 microg/ml bleomycin and chromosomal aberrations in first division metaphases were analysed by fluorescence in situ hybridization (FISH) using arm-specific painting probes for chromosomes 3, 4, 8 and 9. We observed that bleomycin induced all classes of chromosome-type aberrations very efficiently. The interesting findings were: (i) the frequency of induced interstitial translocations (i.e. insertions) was approximately equal to that of reciprocal translocations; (ii) the frequency of induced pericentric inversions was higher than that of centric rings. In our earlier studies, we found that X-rays induced a low frequency of interstitial translocations in comparison with reciprocal translocations and equal frequencies of centric rings and pericentric inversions. These data suggest that bleomycin differs from X-rays with respect to the induction of some specific types of aberrations. The results of a chi2 test examining the hypothesis that formed aberrations among the chromosomes or chromosome arms are randomly distributed on the basis of their relative lengths revealed a differential involvement of these chromosomes in the aberrations following exposure to bleomycin. In general, chromosome 8 was found to be more involved in induced aberrations than expected, chromosome 4 was randomly involved, whereas chromosomes 3 and 9 were less involved. This study demonstrates the utility of arm-specific painting probes for efficient detection of a large variety of chromosomal aberrations induced by bleomycin.
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Authors | Y Xiao, A T Natarajan |
Journal | Mutagenesis
(Mutagenesis)
Vol. 14
Issue 4
Pg. 357-64
(Jul 1999)
ISSN: 0267-8357 [Print] England |
PMID | 10390502
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibiotics, Antineoplastic
- DNA Probes
- Bleomycin
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Topics |
- Animals
- Antibiotics, Antineoplastic
(toxicity)
- Bleomycin
(toxicity)
- Cell Line
- Chromosome Aberrations
- Chromosome Painting
(methods)
- Cricetinae
- Cricetulus
- DNA Probes
- Embryo, Mammalian
- In Situ Hybridization, Fluorescence
- Male
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