Abstract | BACKGROUND: METHODS: A plasmid containing a human calponin h1 complementary DNA and a bacterial neomycin-resistance gene was transfected into the human leiomyosarcoma cell lines SKN and SK-LMS-1 by electroporation. Southern blotting, reverse transcription-polymerase chain reaction analysis, western blotting, and immunohistochemistry were used to confirm DNA transfer and expression of the calponin h1 protein in neomycin-resistant clones. We characterized the morphology of calponin h1-transfected cells, and we evaluated their proliferative activity and tumorigenicity by use of a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay, an anchorage-independent growth assay, and a nude mouse tumorigenicity assay. RESULTS: The morphology of calponin h1-transfected cells in culture resembled that of cultured normal myometrial smooth muscle cells. With SK-LMS-1 cells, proliferation of calponin h1-transfection cells was reduced to 69% of control; with SKN cells, calponin h1 transfection reduced proliferation to 70% of control. In assays of anchorage-independent growth and in vivo tumorigenicity, both growth and tumorigenicity were statistically significantly reduced in calponin h1-transfected leiomyosarcoma cells. CONCLUSIONS:
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Authors | A Horiuchi, T Nikaido, S Taniguchi, S Fujii |
Journal | Journal of the National Cancer Institute
(J Natl Cancer Inst)
Vol. 91
Issue 9
Pg. 790-6
(May 05 1999)
ISSN: 0027-8874 [Print] United States |
PMID | 10328110
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Calcium-Binding Proteins
- DNA, Complementary
- Microfilament Proteins
- Muscle Proteins
- calponin
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Topics |
- Animals
- Blotting, Southern
- Blotting, Western
- Calcium-Binding Proteins
(genetics, metabolism)
- Cell Division
- DNA, Complementary
(metabolism)
- Female
- Humans
- Immunohistochemistry
- In Vitro Techniques
- Leiomyosarcoma
(metabolism, pathology)
- Mice
- Mice, Nude
- Microfilament Proteins
- Muscle Proteins
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Transfection
- Uterine Neoplasms
(metabolism, pathology)
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