Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium.

Abstract

We have identified T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium tumefaciens (rat mutants). These mutants are highly recalcitrant to the induction of both crown gall tumors and phosphinothricin-resistant calli. The results of transient GUS (beta-glucuronidase) assays suggest that some of these mutants are blocked at an early step in the Agrobacterium-mediated transformation process, whereas others are blocked at a step subsequent to translocation of T-DNA into the nucleus. Attachment of Agrobacterium to roots of the mutants rat1 and rat3 was decreased under various incubation conditions. In most mutants, the transformation-deficient phenotype co-segregated with the kanamycin resistance encoded by the mutagenizing T-DNA. In crosses with susceptible wild-type plants, the resistance phenotype of many of these mutants segregated either as a semi-dominant or dominant trait.

• Authors: J Nam, K S Mysore, C Zheng, M K Knue, A G Matthysse, S B Gelvin
• Journal: Molecular & general genetics : MGG (Mol Gen Genet) Vol. 261 Issue 3 Pg. 429-38 (Apr 1999) ISSN: 0026-8925 [Print] Germany
• PMID: 10323222 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA, Bacterial
• T-DNA

Topics

• Agrobacterium tumefaciens (genetics, physiology)
• Arabidopsis (genetics, microbiology)
• Bacterial Adhesion
• Crosses, Genetic
• DNA, Bacterial (genetics)
• Genes, Plant
• Mutation
• Plant Roots (microbiology)
• Transformation, Genetic