Abstract | BACKGROUND: METHODS: Human liver tissue samples from patients with congenital hepatic fibrosis (n = 9) and from normal patients (n = 3) were analyzed. Tissue homogenates from both groups were analyzed for transforming growth factor-beta1 protein and mRNA by Western blot analysis and in situ hybridization, respectively. Immunolocalization studies were performed in fixed tissue sections from both groups. Stellate cells were cultured from livers exhibiting congenital hepatic fibrosis and confirmed by desmin staining. The cells were cultured in serum-free medium for 48 hours, and media were collected and analyzed by Western blot analysis for thrombospondin-1 and transforming growth factor-beta1. RESULTS: CONCLUSIONS:
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Authors | M El-Youssef, Y Mu, L Huang, V Stellmach, S E Crawford |
Journal | Journal of pediatric gastroenterology and nutrition
(J Pediatr Gastroenterol Nutr)
Vol. 28
Issue 4
Pg. 386-92
(Apr 1999)
ISSN: 0277-2116 [Print] United States |
PMID | 10204502
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Culture Media, Conditioned
- Desmin
- RNA, Messenger
- Thrombospondins
- Transforming Growth Factor beta
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Topics |
- Blotting, Western
- Cells, Cultured
- Culture Media, Conditioned
- Desmin
(analysis)
- Gene Expression
- Humans
- In Situ Hybridization
- Liver
(metabolism, pathology)
- Liver Cirrhosis
(congenital, metabolism)
- RNA, Messenger
(analysis)
- Thrombospondins
(analysis, genetics, metabolism)
- Tissue Distribution
- Transforming Growth Factor beta
(analysis, genetics, metabolism)
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