Vaccination with BV8S2 protein amplifies TCR-specific regulation and protection against experimental autoimmune encephalomyelitis in TCR BV8S2 transgenic mice.

Abstract

TCR determinants overexpressed by autopathogenic Th1 cells can naturally induce a second set of TCR-specific regulatory T cells. We addressed the question of whether immune regulation could be induced naturally in a genetically restricted model in which a major portion of TCR-specific regulatory T cells expressed the same target TCR BV8S2 chain as the pathogenic T cells specific for myelin basic protein (MBP). We found vigorous T cell responses to BV8S2 determinants in naive mice that could be further potentiated by vaccination with heterologous BV8S2 proteins, resulting in the selective inhibition of MBP-specific Th1 cells and protection against experimental encephalomyelitis. Moreover, coculture with BV8S2-specific T cells or their supernatants reduced proliferation, IFN-gamma secretion, and encephalitogenic activity of MBP-specific T cells. These results suggest that immune regulation occurs through a nondeletional cytokine-driven suppressive mechanism.

• Authors: H Offner, K Adlard, B F Bebo Jr, J Schuster, G G Burrows, A C Buenafe, A A Vandenbark
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 161 Issue 5 Pg. 2178-86 (Sep 01 1998) ISSN: 0022-1767 [Print] United States
• PMID: 9725209 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Adjuvants, Immunologic
• Epitopes, T-Lymphocyte
• Myelin Basic Protein
• Peptide Fragments
• Receptors, Antigen, T-Cell, alpha-beta
• Freund's Adjuvant

Topics

• Adjuvants, Immunologic (physiology)
• Animals
• Cell Line
• Cell-Free System (immunology)
• Coculture Techniques
• Encephalomyelitis, Autoimmune, Experimental (genetics, immunology, prevention & control)
• Epitopes, T-Lymphocyte (immunology)
• Female
• Freund's Adjuvant (immunology)
• Immunosuppression Therapy
• Lymph Nodes (immunology)
• Lymphocyte Activation (genetics)
• Male
• Mice
• Mice, Inbred C57BL
• Mice, Transgenic
• Myelin Basic Protein (antagonists & inhibitors, genetics, immunology)
• Peptide Fragments (antagonists & inhibitors, genetics, immunology)
• Rats
• Receptors, Antigen, T-Cell, alpha-beta (genetics, immunology, physiology)
• T-Lymphocyte Subsets (immunology)
• Th1 Cells (immunology)
• Transgenes (immunology)
• Vaccination (methods)