Immunoreactivity and in-vitro effect on human sperm-egg binding of antibodies against peptides corresponding to bonnet monkey zona pellucida-3 glycoprotein.

Abstract

The following synthetic peptides were made as immunogens for development of a zona-based contraceptive vaccine: P1, KQPFWLLQGGASRAETSVQPVLVE [amino acids (aa) 23-45 with an additional K at the N-terminus]; P2, FSEEKLVFSLRLMEENC (aa 164-179 with an additional C at the C-terminus and T170 replaced by V); and P3, CSFSKSSNSWFPVEGPADICQCC (aa 300-322). The aa are numbered on the basis of bonnet monkey ZP3 precursor protein. Antibodies against an additional peptide P4, KGDCGTPSHSRRQPHVVSQWSRSA (aa 324-347), significantly inhibits human sperm-oocyte binding. In addition, antibodies against cocktail of peptide-diphtheria toxoid conjugates also significantly inhibit the binding of spermatozoa to zona pellucida in a hemizona assay. These results will further help in the design of an immunocontraceptive vaccine based upon synthetic peptides corresponding to ZP3.

• Authors: A Afzalpurkar, H Shibahara, A Hasegawa, K Koyama, S K Gupta
• Journal: Human reproduction (Oxford, England) (Hum Reprod) Vol. 12 Issue 12 Pg. 2664-70 (Dec 1997) ISSN: 0268-1161 [Print] England
• PMID: 9455832 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Diphtheria Toxoid
• Egg Proteins
• Immune Sera
• Membrane Glycoproteins
• Receptors, Cell Surface
• Vaccines
• ZP3 protein, human
• Zona Pellucida Glycoproteins

Topics

• Amino Acid Sequence
• Animals
• Contraception, Immunologic
• Diphtheria Toxoid (immunology)
• Egg Proteins (chemistry, immunology)
• Female
• Fluorescent Antibody Technique, Indirect
• Humans
• Immune Sera (immunology, pharmacology)
• Immunization
• Macaca radiata (immunology)
• Male
• Membrane Glycoproteins (chemistry, immunology)
• Molecular Sequence Data
• Receptors, Cell Surface
• Sperm-Ovum Interactions (drug effects)
• Vaccines
• Zona Pellucida (immunology)
• Zona Pellucida Glycoproteins