Abstract |
Chromosome 13 is one of the poorly mapped human chromosomes. As an example, only two cloned genes have been assigned to bands 13q22-q31. Our characterization of the critical region for the variant form of late infantile neuronal ceroid lipofuscinosis ( vLINCL, locus definition CLN5) disease region on 13q22 resulted in the identification of the sequences encoding the BTF3 protein homologue gene (HGMW-approved symbol BTF3) and a novel pseudogene for RNA Helicase A (HGMW-approved symbol DDX9P). Precise visual assignment to the physical clones covering this region and the positional relationships of these genes were achieved by the use of tyramine enhancement of Fiber-FISH hybridization signals, demonstrating the power of this technique in efficient positioning of coding regions on the physical maps.
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Authors | T Klockars, J Isosomppi, M Laan, N Kakko, A Palotie, L Peltonen |
Journal | Genomics
(Genomics)
Vol. 44
Issue 3
Pg. 355-7
(Sep 15 1997)
ISSN: 0888-7543 [Print] United States |
PMID | 9325059
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Nuclear Proteins
- Transcription Factors
- transcription factor BTF3
- RNA Nucleotidyltransferases
- RNA Helicases
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Topics |
- Chromosomes, Human, Pair 13
- Humans
- In Situ Hybridization, Fluorescence
(methods)
- Nuclear Proteins
- Pseudogenes
- RNA Helicases
- RNA Nucleotidyltransferases
(genetics)
- Transcription Factors
(genetics)
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