Adaptation of the primer extension preamplification (PEP) reaction for preimplantation diagnosis: single blastomere analysis using short PEP protocols.

Abstract

Primer extension preamplification (PEP) was first described as a method for whole genome amplification, starting from a single cell, originally a spermatozoon, in order to perform genetic recombination studies. Its usefulness for preimplantation diagnosis was shown soon after; the only drawback was the length of the procedure (> 14 h). We have developed a shorter PEP protocol for single human blastomeres, enabling us to examine several genetic loci of interest in human genetic diseases with a good amplification efficiency.

• Authors: K Sermon, W Lissens, H Joris, A Van Steirteghem, I Liebaers
• Journal: Molecular human reproduction (Mol Hum Reprod) Vol. 2 Issue 3 Pg. 209-12 (Mar 1996) ISSN: 1360-9947 [Print] England
• PMID: 9238681 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• DNA Primers

Topics

• Blastomeres (physiology)
• Cystic Fibrosis (genetics)
• DNA Primers
• Embryonic Development
• Female
• Fertilization in Vitro (methods)
• Humans
• Polymerase Chain Reaction (methods)
• Pregnancy
• Tay-Sachs Disease (genetics)