Abstract |
The murine AIDS ( MAIDS) is a retrovirus-induced disease that shows severe immunodeficiency with abnormal lymphoproliferation in susceptible strains of mice. To clarify the antigenicity of gag gene products of the LP-BM5 defective virus, which is known as the causative virus of MAIDS, we expressed and purified the gag p12 gene product (P12) by using a baculovirus expression vector system. The P12 protein strongly stimulated the proliferation of normal C57BL/6 (B6) lymph node T-cells in vitro. Furthermore, a 25-mer synthetic polypeptide within the P12 sequence gave rise to the similar or even higher activation of T-cells. The phenotype of responding T-cells was found to be CD8+ CD44low, indicating that naive CD8+ T-cells respond against a peptide encoded within a MAIDS defective virus gag p12 gene. Finally, the expression of T-cell receptor (TcR) V beta on the responding CD8+ T-cells was analyzed. Although CD8+ T-cells with the particular V beta chains were expanded in response to the 25-mer peptide, this polypeptide does not seem to be a superantigen, since this response is MHC class I-restricted and the V beta preference is not striking. The presentation pathway of this highly antigenic polypeptide will be discussed.
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Authors | S T Yee, E Abe, Y Okada, Y Matsuura, Y Takebe, K Ogasawara, H Takahashi, T Mizuochi |
Journal | Immunology letters
(Immunol Lett)
Vol. 55
Issue 2
Pg. 93-8
(Feb 1997)
ISSN: 0165-2478 [Print] Netherlands |
PMID | 9143939
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Gene Products, gag
- Histocompatibility Antigens Class I
- Hyaluronan Receptors
- Peptides
- Receptors, Antigen, T-Cell, alpha-beta
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Topics |
- Amino Acid Sequence
- Animals
- CD8-Positive T-Lymphocytes
(cytology, drug effects, immunology)
- Cell Division
- Cell Line
- Cells, Cultured
- Defective Viruses
(chemistry)
- Gene Products, gag
(genetics, immunology, pharmacology)
- Histocompatibility Antigens Class I
(immunology)
- Hyaluronan Receptors
(immunology)
- Leukemia Virus, Murine
(chemistry)
- Lymph Nodes
(cytology)
- Mice
- Mice, Inbred C57BL
- Molecular Sequence Data
- Peptides
(genetics, immunology, pharmacology)
- Receptors, Antigen, T-Cell, alpha-beta
(immunology)
- Spodoptera
(cytology)
- Tumor Cells, Cultured
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