Abstract |
The synthesis and purification of [13C2] p-hydroxyphenyllactic acid from [13C2]p-hydroxyphenylpyruvic acid, the characterization of tert.-butyldimethylsilyl-derivatized tyrosine, p-hydroxyphenylpyruvic acid and p-hydroxyphenyllactic acid, and an isotope-dilution assay for these substances in normal human plasma using gas chromatography-mass spectrometry (GC-MS) are described. Using this method plasma p-hydroxyphenylpyruvate, p-hydroxyphenyllactate and tyrosine levels of 68 +/- 42 ng/ml, 118 +/- 45 ng/ml and 16.6 +/- 6.3 micrograms/ml, respectively, were found in 9 normal adults. Isotope-dilution assays are sensitive enough to determine tyrosine, p-hydroxyphenylpyruvate and p-hydroxyphenyllactate content in normal subjects, and may be useful for studying disorders of tyrosine metabolism, including inborn errors of metabolism, liver disease and ascorbic acid deficiencies.
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Authors | J C Deutsch |
Journal | Journal of chromatography. B, Biomedical sciences and applications
(J Chromatogr B Biomed Sci Appl)
Vol. 690
Issue 1-2
Pg. 1-6
(Mar 07 1997)
ISSN: 1387-2273 [Print] Netherlands |
PMID | 9106023
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Carbon Isotopes
- Phenylpropionates
- Phenylpyruvic Acids
- 4-hydroxyphenylpyruvic acid
- 4-hydroxyphenyllactic acid
- Tyrosine
- Deuterium
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Topics |
- Carbon Isotopes
- Deuterium
- Gas Chromatography-Mass Spectrometry
- Humans
- Indicator Dilution Techniques
- Phenylpropionates
(blood)
- Phenylpyruvic Acids
(blood)
- Tyrosine
(blood)
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