Localization of histidyl-tRNA synthetase (Jo-1) in human laryngeal epithelial carcinoma cell line (HEp-2 cells).

Abstract

The present study was designed to determine the subcellular localization of histidyl-tRNA synthetase (Jo-1) in human laryngeal epithelial carcinoma cell line (HEp-2 cells). Indirect immunofluorescence using commercial HEp-2 cells with human serum and human-affinity-purified anti-Jo-1 antibodies was performed using confocal microscopy. Anti-histidyl-tRNA-synthetase-positive sera showed distinct nuclear and cytoplasmic granular staining in HEp-2 cells. Affinity purified anti-Jo-1 produced an identical pattern to the whole serum, whereas the serum fraction that did not bind to the affinity column was negative by immunofluorescence on HEp-2 cells. Two commercial human anti-Jo-1-positive control sera and seven anti-Jo-1-positive sera from patients with myositis reproduced the nuclear and cytoplasmic granular pattern. We conclude that Jo-1 is present in cytoplasm and in intact nuclei from HEp-2 cells. The presence of tRNA synthetases in intact nuclei suggests that they have an unsuspected function in the nucleus.

• Authors: D Vázquez-Abad, J H Carson, N Rothfield
• Journal: Cell and tissue research (Cell Tissue Res) Vol. 286 Issue 3 Pg. 487-91 (Dec 1996) ISSN: 0302-766X [Print] Germany
• PMID: 8929351 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Histidine-tRNA Ligase

Topics

• Cell Nucleus (metabolism)
• Cytoplasm (metabolism)
• HeLa Cells
• Histidine-tRNA Ligase (metabolism)
• Humans
• Laryngeal Neoplasms (pathology)
• Myositis (blood, immunology)
• Tumor Cells, Cultured