Rapid differentiation of Mycobacterium avium and M. paratuberculosis by PCR and restriction enzyme analysis.

Abstract

Mycobacterium avium subsp. avium (M. avium) and M. avium subsp. paratuberculosis (M. paratuberculosis), intracellular bacteria that can cause chronic granulomatous enteritis in cattle, are difficult to distinguish on the basis of growth and biochemical characteristics. We report the development of a PCR-based strategy for the rapid differentiation of isolates of M. avium from isolates of M. paratuberculosis. Restriction fragment length polymorphism was identified by PCR amplification and subsequent restriction enzyme digestion with PstI of a 960-bp fragment of the 65-kDa heat shock protein (hsp65) from 21 clinical isolates of M. paratuberculosis and 14 isolates of M. avium. These results indicate that a restriction fragment length polymorphism in the hsp65 gene can be used for the rapid differentiation of clinical isolates of M. paratuberculosis and M. avium.

• Authors: I S Eriks, K T Munck, T E Besser, G H Cantor, V Kapur
• Journal: Journal of clinical microbiology (J Clin Microbiol) Vol. 34 Issue 3 Pg. 734-7 (Mar 1996) ISSN: 0095-1137 [Print] United States
• PMID: 8904449 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• DNA, Bacterial

Topics

• Animals
• Base Sequence
• DNA, Bacterial (chemistry)
• Humans
• Molecular Sequence Data
• Mycobacterium avium (genetics, isolation & purification)
• Mycobacterium avium subsp. paratuberculosis (genetics, isolation & purification)
• Polymerase Chain Reaction
• Polymorphism, Restriction Fragment Length