Abstract | BACKGROUND & AIMS: METHODS: Rats received NOS inhibitors or NO donors before measurement of toxin-mediated ileal secretion and permeability changes. Mucosal mast cell and neutrophil activity were measured by release of rat mast cell protease II and myeloperoxidase activity, respectively. RESULTS: NOS inhibitors augmented but an NO donor inhibited toxin A-mediated ileal secretion and permeability when given before but not after toxin administration. Neither an NOS inhibitor nor an NO donor had any effect on cholera toxin-mediated secretion. Mast cell degranulation and neutrophil infiltration occurred after injection of toxin A or an NOS inhibitor, whereas the NO donor blocked both toxin A effects. CONCLUSIONS: NOS inhibitors augmented and an NO donor blocked the intestinal effects of toxin A but not of cholera toxin. NO protects against toxin A by inhibition of intestinal mast cells and neutrophils, which are activated by toxin A, but not by cholera toxin.
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Authors | B Qiu, C Pothoulakis, I Castagliuolo, Z Nikulasson, J T LaMont |
Journal | Gastroenterology
(Gastroenterology)
Vol. 111
Issue 2
Pg. 409-18
(Aug 1996)
ISSN: 0016-5085 [Print] United States |
PMID | 8690206
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Bacterial Toxins
- Enterotoxins
- Enzyme Inhibitors
- Guanidines
- Indazoles
- stN protein, Vibrio cholerae
- tcdA protein, Clostridium difficile
- Nitric Oxide
- S-nitroso-N-acetylcysteine
- Arginine
- Peroxidase
- Nitric Oxide Synthase
- Serine Endopeptidases
- chymase 2
- Chymases
- pimagedine
- 7-nitroindazole
- NG-Nitroarginine Methyl Ester
- Acetylcysteine
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Topics |
- Acetylcysteine
(analogs & derivatives, pharmacology)
- Animals
- Arginine
(analogs & derivatives, pharmacology)
- Bacterial Toxins
- Cell Degranulation
(drug effects)
- Chymases
- Clostridioides difficile
(metabolism)
- Enterotoxins
(pharmacology)
- Enzyme Inhibitors
(pharmacology)
- Guanidines
(pharmacology)
- Ileum
(cytology, drug effects, metabolism)
- Indazoles
(pharmacology)
- Intestinal Mucosa
(cytology, drug effects, metabolism)
- Male
- Mast Cells
(drug effects, enzymology, physiology)
- NG-Nitroarginine Methyl Ester
- Neutrophils
(drug effects, enzymology, physiology)
- Nitric Oxide
(physiology)
- Nitric Oxide Synthase
(antagonists & inhibitors)
- Permeability
- Peroxidase
(metabolism)
- Rats
- Rats, Wistar
- Serine Endopeptidases
(metabolism)
- Vibrio cholerae
(metabolism)
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