Detection of c-mos proto-oncogene expression in human cells.

Abstract

Although the human c-mos proto-oncogene has been characterized for more than a decade, very little is known about its protein product and its expression in somatic cells. We generated three human c-mos-specific antisera and report here the detection of c-mos protein in a human neuroblastoma cell line, SK-N-BE2 (BE2). Both Western (immuno-) blot and immunoprecipitation analyses detected a p37 as the major form and p40 and p35 as minor forms of the c-mos protein. Using Northern blot analysis, 3.5- and 1.7-kb c-mos messages were detected. Using a highly sensitive method that combines reverse transcription and the polymerase chain reaction (RT-PCR), c-mos RNA was detected in all the human samples examined. With Western blot analysis, we further showed that c-mos proteins are expressed in cervical carcinoma-derived cell lines. This ubiquitous expression of low levels of c-mos suggests a fundamental role for the c-mos proto-oncogene.

• Authors: C C Li, E Chen, C D O'Connell, D L Longo
• Journal: Oncogene (Oncogene) Vol. 8 Issue 6 Pg. 1685-91 (Jun 1993) ISSN: 0950-9232 [Print] England
• PMID: 8502488 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• MAS1 protein, human
• Oligodeoxyribonucleotides
• Proto-Oncogene Mas
• RNA, Neoplasm
• Proto-Oncogene Proteins c-mos

Topics

• Animals
• Base Sequence
• Blotting, Northern
• Blotting, Western
• Cell Line
• Electrophoresis, Polyacrylamide Gel
• Female
• Gene Expression
• Genes, mos
• Humans
• Mice
• Molecular Sequence Data
• Neuroblastoma
• Oligodeoxyribonucleotides
• Polymerase Chain Reaction
• Proto-Oncogene Mas
• Proto-Oncogene Proteins c-mos (analysis, biosynthesis, genetics)
• RNA, Neoplasm (analysis, genetics)
• Tumor Cells, Cultured
• Uterine Cervical Neoplasms