Abstract |
The growth and differentiation potential of Y79 human retinoblastoma cells was assessed in vitro following treatment with the differentiating agent succinylated concanavalin A (SCA). Since SCA treatment induced Y79 cells to display differentiated morphologies in vitro, we sought to determine potential differentiated phenotypes with the use of retinal cell markers. Seventy-two h after SCA treatment, Y79 cells exhibited a decrease in the glial cell marker GFAP and a dramatic and reversible increase in the photoreceptor marker IRBP, while maintaining neuron-specific enolase and PGP 9.5 positivity. These results were indicative of a predominantly neuronal, photoreceptor cell population in response to SCA treatment. In addition, Y79 cell growth inhibition was observed in response to SCA, which could be reversed within 24 h of treatment with the blocking sugar alpha-methyl-D- mannoside. These changes were accompanied by a significant modulation of the N-MYC oncoprotein, as detected by Western blot analysis and immunocytochemistry. Thus, in this system, the status of N-MYC seems to be closely linked to changes in the growth and differentiated state of SCA-treated Y79 retinoblastoma cells.
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Authors | G M Seigel, M F Notter |
Journal | Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
(Cell Growth Differ)
Vol. 4
Issue 1
Pg. 1-7
(Jan 1993)
ISSN: 1044-9523 [Print] United States |
PMID | 8424902
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Eye Proteins
- Proto-Oncogene Proteins c-myc
- Retinol-Binding Proteins
- interstitial retinol-binding protein
- Concanavalin A
- succinylconcanavalin A
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Topics |
- Cell Differentiation
(drug effects)
- Cell Division
(drug effects)
- Concanavalin A
(therapeutic use)
- Eye Proteins
(biosynthesis)
- Humans
- Mitotic Index
- Neuroglia
(drug effects)
- Neurons
(drug effects)
- Phenotype
- Proto-Oncogene Proteins c-myc
(biosynthesis)
- Retinoblastoma
(drug therapy, metabolism, pathology)
- Retinol-Binding Proteins
(biosynthesis)
- Tumor Cells, Cultured
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