Abstract | BACKGROUND: METHODS: The homogeneity of yeast sucrase was studied by sodium dodecyl sulfate- polyacrylamide gel electrophoresis, and its activity was measured. Stability at various temperatures and pH ranges and in the presence of gastric aspirate, pepsin, and bovine serum albumin was assessed. Fourteen patients with CSID underwent double-blind placebo-controlled breath tests with yeast sucrase. They then completed an 8-week dose response study that used different enzyme concentrations while consuming a sucrose-containing diet. RESULTS: Liquid yeast sucrase is highly glycosylated, contains no lactase activity, and is stable at 4 degrees C and over a wide range of pH. Pepsin digestion of the enzyme in vitro can be blunted by bovine serum albumin and by increasing the pH. Yeast sucrase reduces breath hydrogen excretion in patients with CSID who are given a sucrose load (P < 0.001) and allows most patients to consume a sucrose-containing diet. CONCLUSIONS:
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Authors | W R Treem, N Ahsan, B Sullivan, T Rossi, R Holmes, J Fitzgerald, R Proujansky, J Hyams |
Journal | Gastroenterology
(Gastroenterology)
Vol. 105
Issue 4
Pg. 1061-8
(Oct 1993)
ISSN: 0016-5085 [Print] United States |
PMID | 8405850
(Publication Type: Clinical Trial, Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Serum Albumin
- Sucrose
- Hydrogen
- Sucrase-Isomaltase Complex
- Sucrase
- Pepsin A
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Topics |
- Administration, Oral
- Adolescent
- Adult
- Analysis of Variance
- Carbohydrate Metabolism, Inborn Errors
(drug therapy, metabolism)
- Child
- Child, Preschool
- Dose-Response Relationship, Drug
- Double-Blind Method
- Electrophoresis, Polyacrylamide Gel
- Female
- Humans
- Hydrogen
(analysis, metabolism)
- Hydrogen-Ion Concentration
- Infant
- Male
- Pepsin A
(pharmacology)
- Saccharomyces cerevisiae
(enzymology)
- Serum Albumin
(pharmacology)
- Sucrase
(administration & dosage, metabolism, therapeutic use)
- Sucrase-Isomaltase Complex
(deficiency)
- Sucrose
(administration & dosage, pharmacology)
- Temperature
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