Abstract |
We have developed radioimmunoassays for the quantification of apolipoproteins ( apo) C-I, C-II, and C-III in human plasma. The apo C proteins were isolated from very low-density lipoproteins of patients with hypertriglyceridemia, fractionated on a Sephacryl column, and purified by diethylaminoethyl cellulose anion-exchange chromatography followed by reverse-phase fast protein liquid chromatography. The assays were sensitive, specific, and reproducible, and the standards demonstrated parallel immunoreactivity with plasma samples. Patients with hypertriglyceridemia ( triglyceride level more than 2,200 mg/liter)--14 patients with diabetes and 12 with type V hyperlipoproteinemia--were compared with age- and sex-matched control subjects. In comparison with the control groups, levels of apoproteins C-I, C-II, and C-III were significantly increased in both disease groups, but the ratios of the C peptides to triglycerides were significantly lower, an indication of a relative deficiency of C apoproteins in hypertriglyceridemic states. Independent radioimmunoassays for each of the C apolipoproteins would help to study their individual roles in triglyceride-rich lipoprotein metabolism.
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Authors | N D Bren, A Rastogi, B A Kottke |
Journal | Mayo Clinic proceedings
(Mayo Clin Proc)
Vol. 68
Issue 7
Pg. 657-64
(Jul 1993)
ISSN: 0025-6196 [Print] England |
PMID | 8350639
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Apolipoprotein C-I
- Apolipoprotein C-II
- Apolipoprotein C-III
- Apolipoproteins C
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Topics |
- Adult
- Aged
- Apolipoprotein C-I
- Apolipoprotein C-II
- Apolipoprotein C-III
- Apolipoproteins C
(blood)
- Diabetes Mellitus
(blood)
- Female
- Humans
- Hyperlipoproteinemia Type IV
(blood)
- Hyperlipoproteinemia Type V
(blood)
- Male
- Middle Aged
- Radioimmunoassay
(methods)
- Reproducibility of Results
- Sensitivity and Specificity
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