Two sites of action for LCB29 (idrocilamide) in depressing mechanical tension of rat soleus muscle fibers?

Abstract	The effects of 50 microM LCB29 (idrocilamide) were tested on depolarization-induced and caffeine contractures of rat soleus muscle fibers. When applied intracellularly by free diffusion in cut-end voltage-clamped fibers, LCB29 decreased tension amplitude by about 25%. The same amount of inhibition by LCB29 was observed on contractures induced by 6 mM caffeine. The drug did not affect the repriming of caffeine contractures, indicating that internal recycling of calcium was not affected. The voltage-dependent inactivation of tension was facilitated by external application of LCB29. This effect was calcium dependent, so that the greater the external calcium concentration, the greater the drug effectiveness. The spontaneous relaxation of K+ contractures was also accelerated by LCB29. It is concluded that LCB29 acts intracellularly by decreasing sarcoplasmic reticulum calcium release and externally by facilitating the voltage-dependent inactivation of the voltage sensor for excitation-contraction coupling.
Authors	A Mouzou, A Bouron, J Guillemain, D Guerrier, G Raymond
Journal	Canadian journal of physiology and pharmacology (Can J Physiol Pharmacol) Vol. 71 Issue 12 Pg. 889-95 (Dec 1993) ISSN: 0008-4212 [Print] Canada
PMID	8180884 (Publication Type: Journal Article)
Chemical References	Calcium Channels Ethanolamines Muscle Relaxants, Central Caffeine idrocilamide Sodium Potassium Calcium
Topics	Animals Caffeine (pharmacology) Calcium (metabolism, physiology) Calcium Channels (drug effects) Depression, Chemical Electric Stimulation Ethanolamines (pharmacology) Female In Vitro Techniques Muscle Contraction (drug effects) Muscle Relaxants, Central (pharmacology) Muscle Relaxation (drug effects) Muscles (cytology, drug effects) Potassium (pharmacology) Rats Rats, Wistar Sarcoplasmic Reticulum (drug effects, metabolism) Sodium (metabolism, physiology)

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