Detection of Borrelia burgdorferi antigens in cerebrospinal fluid.

Abstract

We examined CSF for Borrelia burgdorferi antigens using antigen-capture ELISA and Western (immuno) blot. Antigen-capture ELISA was positive in 38 of 77 (49%) CSF samples obtained from neurologic patients with presumed B burgdorferi infection, compared with one of 34 (3%) CSF samples obtained from other neurologic disease controls who came from a region endemic for Lyme disease. Western immunoblot was positive for B burgdorferi antigens in 12 of 22 (55%) CSF samples from the B burgdorferi infected groups, compared with none of 11 CSF samples from the control group. CSF antigen detection should prove helpful in evaluating patients for suspected neurologic Lyme disease.

• Authors: P K Coyle, Z Deng, S E Schutzer, A L Belman, J Benach, L B Krupp, B Luft
• Journal: Neurology (Neurology) Vol. 43 Issue 6 Pg. 1093-8 (Jun 1993) ISSN: 0028-3878 [Print] United States
• PMID: 8170548 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Bacterial

Topics

• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Antigens, Bacterial (cerebrospinal fluid)
• Blotting, Western
• Borrelia burgdorferi Group (immunology)
• Child
• Child, Preschool
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Lyme Disease (cerebrospinal fluid)
• Male
• Middle Aged
• Sensitivity and Specificity