Cloning, sequencing, and mutational analysis of the hyb operon encoding Escherichia coli hydrogenase 2.

Abstract

The genes encoding the two structural subunits of Escherichia coli hydrogenase 2 (HYD2) have been cloned and sequenced. They occur in an operon (hyb) which contains seven open reading frames. An hyb deletion mutant (strain AP3) failed to grown on dihydrogen-fumarate medium and also produced very low levels of HYD1. All seven open reading frames are required for restoration of wild-type levels of active HYD2 in AP3. The hyb operon was mapped at 65 min on the E. coli chromosome.

• Authors: N K Menon, C Y Chatelus, M Dervartanian, J C Wendt, K T Shanmugam, H D Peck Jr, A E Przybyla
• Journal: Journal of bacteriology (J Bacteriol) Vol. 176 Issue 14 Pg. 4416-23 (Jul 1994) ISSN: 0021-9193 [Print] United States
• PMID: 8021226 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Hydrogenase

Topics

• Amino Acid Sequence
• Base Sequence
• Cloning, Molecular
• DNA Mutational Analysis
• Escherichia coli (enzymology, genetics)
• Genes, Bacterial
• Genetic Complementation Test
• Hydrogenase (chemistry, genetics, metabolism)
• Molecular Sequence Data
• Operon
• Phenotype
• Sequence Analysis, DNA