Failure of pentoxifylline to ameliorate severe acute pancreatitis in the rat: results of a prospective, randomized, controlled study.

Abstract

OBJECTIVE:

To investigate the benefit of pentoxifylline in severe experimental pancreatitis.

DESIGN:

Prospective, randomized, controlled study.

SETTING:

Experimental animal laboratory in a University hospital.

SUBJECTS:

Forty-two adult male Sprague-Dawley rats.

INTERVENTIONS:

Acute pancreatitis was induced by supramaximal stimulation with cerulein plus a pressure and volume controlled 10 min intraductal infusion of 10-mM glycodeoxycholic acid. Thirty minutes after pancreatitis was induced, animals were randomized to receive pentoxifylline (60 mg/kg over 2.5 hrs), or saline. All animals received fluid resuscitation with lactated Ringer's solution (8 mL/kg/hr), and surviving animals were killed at 24 hrs.

MEASUREMENTS AND MAIN RESULTS:

There was a progressively significant decrease in mean arterial pressure after pancreatitis was induced, with no difference between pentoxifylline-treated rats and controls. Hematocrit increased significantly in both groups at 6 hrs, and returned to baseline values at 24 hrs. Ascites volume and levels of trypsinogen activation peptide in plasma and ascites were similar in both groups. Twenty-four hour mortality was 47% for the pentoxifylline group and 52% for the control group. Histologic scores for necrosis, edema, inflammation, and hemorrhage showed no significant differences between the two groups.

CONCLUSION:

Treatment with pentoxifylline failed to improve outcome in a model of severe acute pancreatitis in the rat.

• Authors: D G Bassi, T Foitzik, D W Rattner, K Lewandrowski, A L Warshaw, C Fernández-del Castillo
• Journal: Critical care medicine (Crit Care Med) Vol. 22 Issue 12 Pg. 1960-3 (Dec 1994) ISSN: 0090-3493 [Print] United States
• PMID: 7988133 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Glycodeoxycholic Acid
• Ceruletide
• Pentoxifylline

Topics

• Acute Disease
• Analysis of Variance
• Animals
• Ceruletide
• Chi-Square Distribution
• Disease Models, Animal
• Drug Evaluation, Preclinical
• Glycodeoxycholic Acid
• Male
• Pancreas (pathology)
• Pancreatitis (chemically induced, drug therapy, pathology)
• Pentoxifylline (therapeutic use)
• Prospective Studies
• Random Allocation
• Rats
• Rats, Sprague-Dawley