Orphan receptor COUP-TF I antagonizes retinoic acid-induced neuronal differentiation.

Abstract

Chicken ovalbumin upstream promoter-transcription factors (COUP-TF) are expressed in the developing nervous system and interact with nuclear hormone receptors to regulate expression of different genes. The role of COUP-TF orphan receptors in neurogenesis is virtually unknown. To study the possible function of COUP-TF I during neuronal differentiation, we generated COUP-TF I overexpressing teratocarcinoma PCC7 cell lines and analyzed retinoic acid (RA)-induced neuronal differentiation of these cells. COUP-TF I overexpression results in the blockade of morphological differentiation after induction to differentiate. COUP-TF I represses expression of microtubule-associated protein 2 (MAP2) gene and delays induction of growth-associated protein 43 (GAP43) gene expression. In contrast, expression of the neurofilament light subunit (NF-L) gene is not affected by COUP-TF I overexpression during neuronal differentiation. Also, cells overexpressing COUP-TF I do not stop proliferating after RA and dBcAMP treatment and possess suppressed transcriptional activation from different RA response elements. These results suggest that COUP-TF I plays an important role in regulating RA-induced neuronal differentiation.

• Authors: K Neuman, A Soosaar, H O Nornes, T Neuman
• Journal: Journal of neuroscience research (J Neurosci Res) Vol. 41 Issue 1 Pg. 39-48 (May 01 1995) ISSN: 0360-4012 [Print] United States
• PMID: 7674376 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers
• COUP Transcription Factor I
• DNA-Binding Proteins
• GAP-43 Protein
• Membrane Glycoproteins
• Microtubule-Associated Proteins
• Nerve Tissue Proteins
• Neurofilament Proteins
• Nr2f1 protein, mouse
• Receptors, Glucocorticoid
• Transcription Factors
• Tretinoin
• Bucladesine

Topics

• Animals
• Base Sequence
• Biomarkers
• Bucladesine (pharmacology)
• COUP Transcription Factor I
• Cell Cycle (drug effects, genetics)
• Cell Differentiation (drug effects, physiology)
• DNA-Binding Proteins (genetics, pharmacology)
• Enhancer Elements, Genetic (genetics)
• GAP-43 Protein
• Gene Expression (physiology)
• Membrane Glycoproteins (genetics)
• Mice
• Microtubule-Associated Proteins (genetics)
• Molecular Sequence Data
• Nerve Tissue Proteins (genetics)
• Neurofilament Proteins (genetics)
• Receptors, Glucocorticoid (physiology)
• Teratocarcinoma (pathology, physiopathology)
• Transcription Factors (genetics, pharmacology)
• Tretinoin (pharmacology)
• Tumor Cells, Cultured (cytology)