Changes in the cellular glycosaminoglycans of cultured mastocytoma cells induced by sodium butyrate.

Abstract

The effect of sodium butyrate on the cellular glycosaminoglycans of cultured mastocytoma p-815-4 cells was investigated using enzymic digestion, electrophoresis, nitrous acid degradation, and sequential partition fractionation. The average cellular glycosaminoglycan content of mastocytoma p-815-4 cells grown in the presence of 2 mM sodium butyrate was ten times as much as that of the control p-815-4 cells. Approximately 90% of the glycosaminoglycans isolated from the control cells and 70% from the butyrate-treated cells were found to be chondroitin 4-sulfate by enzymic digestion. The remainders were chondroitinase ABC-resistant. Hyaluronic acid and dermatan sulfate were not detected in either control cells or butyrate-treated cells. The chondroitinase ABC-resistant fraction of glycosaminoglycans from butyrate-treated cells showed a molar ratio of sulfate to uronic acid of more than 2.0, and provided some physicochemical properties characteristic to reference bovine lung heparin.

• Authors: N Nakamura, Y Mori, Y Tanigaki, J Kojima
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 627 Issue 1 Pg. 60-70 (Jan 03 1980) ISSN: 0006-3002 [Print] Netherlands
• PMID: 6766327 (Publication Type: Journal Article)

Chemical References

• Butyrates
• Glycosaminoglycans
• Dermatan Sulfate
• Hyaluronic Acid
• Chondroitin Sulfates
• Nitrous Acid

Topics

• Animals
• Butyrates (pharmacology)
• Cells, Cultured
• Chondroitin Sulfates (metabolism)
• Dermatan Sulfate (metabolism)
• Glycosaminoglycans (metabolism)
• Hyaluronic Acid (metabolism)
• Mast-Cell Sarcoma (metabolism)
• Mice
• Neoplasms, Experimental (metabolism)
• Nitrous Acid