Abstract |
The productively rearranged immunoglobulin mu chain gene and the translocated cellular oncogene c-myc are transcribed at high levels both in human Burkitt lymphoma cells carrying the t(8;14) chromosome translocation and in mouse plasmacytoma X Burkitt lymphoma cell hybrids. In the experiments reported here these genes were found to be repressed in mouse 3T3 fibroblast X Burkitt lymphoma cell hybrids. Such repression probably occurs at the transcriptional level since no human mu- and c-myc messenger RNA's are detectable in hybrid clones carrying the corresponding genes. It is therefore concluded that the ability to express these genes requires a differential B cell environment. The results suggest that the 3T3 cell assay may not be suitable to detect oncogenes directly involved in human B cell oncogenesis, since 3T3 cells apparently are incapable of transcribing an oncogene that is highly active in malignant B cells with specific chromosomal translocations.
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Authors | K Nishikura, A ar-Rushdi, J Erikson, E DeJesus, D Dugan, C M Croce |
Journal | Science (New York, N.Y.)
(Science)
Vol. 224
Issue 4647
Pg. 399-402
(Apr 27 1984)
ISSN: 0036-8075 [Print] United States |
PMID | 6424234
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Immunoglobulin Heavy Chains
- Immunoglobulin mu-Chains
- RNA, Messenger
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Topics |
- Animals
- Burkitt Lymphoma
(genetics)
- Fibroblasts
- Gene Expression Regulation
- Genes
- Humans
- Hybrid Cells
(metabolism)
- Immunoglobulin Heavy Chains
(genetics)
- Immunoglobulin mu-Chains
(genetics)
- Mice
- Oncogenes
- RNA, Messenger
(genetics)
- Transcription, Genetic
- Translocation, Genetic
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