Inhibition of IgE production in B hybridomas by IgE class-specific suppressor factor from T hybridomas.
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| Abstract |
The function of IgE class-specific suppressor factor (IgE-TsF) from T hybridomas was studied by employing IgE-producing B hybridomas. IgE-TsF was obtained from IgE class-specific T hybridomas, which had been established by the fusion of a phosphorylcholine-conjugated Mycobacterium-primed T cell population with the T lymphoma cell line BW5147. The absorption experiments showed that IgE-TsF from T hybridomas was composed of the binding site(s) for IgE and I region gene products as observed in conventional IgE-TsF. Incubation of IgE-producing B hybridomas with IgE-TsF for 1 hr at 37 degrees C resulted in the reduction of the number of IgE-secreting cells when assessed by a reverse plaque assay. The proportions of surface IgE-positive cells were concomitantly reduced. After 24 hr incubation with IgE-TsF, the number of cytoplasmic IgE-positive cells was reduced, showing that IgE synthesis was inhibited by IgE-TsF. Antigen-specific TsF from phosphorylcholine-specific T hybridomas did not show any inhibitory effect, and IgE-TsF did not block the antibody production of IgM-producing B hybridomas. Precapping of IgE receptors by anti-epsilon antibody or the simultaneous addition of soluble IgE with IgE-TsF abrogated the suppressive function, suggesting that IgE-TsF acted directly on B epsilon cells through binding with IgE receptors.
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| Authors | M Suemura, A Ishizaka, S Kobatake, K Sugimura, K Maeda, K Nakanishi, S Kishimoto, Y Yamamura, T Kishimoto |
| Journal | Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 130 Issue 3 Pg. 1056-60 (Mar 1983) ISSN: 0022-1767 [Print] United States |
| PMID | 6218197 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't) |
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