Immunological studies in combined factor V and factor VIII deficiency.

Abstract

Plasma samples from patients with inherited combined factor V and factor VIII deficiency were examined by immunological methods for the presence of factor V and Factor VIII-related antigens. A factor V-related antigen was consistently demonstrated in all plasma samples by inhibitor neutralization assay using a non-precipitating rabbit antibody. Factor VIII-related antigens were detected by inhibitor neutralization using human antibody and by electroimmunoassay using a precipitating rabbit antibody. The amounts of factor V and factor VIII-related antigens present in the patient's samples were similar to those found in normal human plasma. The findings confirm the presence of normal levels of factor VIII-related antigen in the plasma of these patients and suggest that inactive antigenic determinants of procoagulant factor V and procoagulant factor VIII are also present. The results are consistent with the possibility that a common precursor of porcoagulant factor V and factor VIII is defective in these patients.

• Authors: J C Giddings, U Seligsohn, A L Bloom
• Journal: British journal of haematology (Br J Haematol) Vol. 37 Issue 2 Pg. 257-64 (Oct 1977) ISSN: 0007-1048 [Print] England
• PMID: 603759 (Publication Type: Journal Article)

Chemical References

• Antigens
• Factor V
• Factor VIII

Topics

• Antigens (analysis)
• Factor V (immunology)
• Factor V Deficiency (complications, immunology)
• Factor VIII (immunology)
• Hemophilia A (complications, immunology)
• Humans
• Neutralization Tests