[Fructose utilization by hepatocytes isolated from fed obese fa/fa Zucker rats. Metabolic fate of fructose in the absence of the modulator].

Abstract

The utilization of fructose by isolated hepatocytes was investigated in fed obese Zucker fa/fa rats compared with their lean littermates (Fa/?) and Sprague-Dawley rats. Hepatocytes were incubated during 3 h using U14C fructose (20 mM). Our results show: a significant increase of fructose consumption, glucose, lactate and pyruvate production and faster turnover of glycogen by fa/fa rats. In these animals, synthesis of acylglycerol was also significantly enhanced. Our results suggest that fructose in fa/fa rats was used preferentially as precursor for lipid synthesis not only by the liver but also by the adipose tissue after a prior transformation into glucose by hepatocytes. All these abnormalities result in an accumulation of acylglycerols maintaining an obesity state in fa/fa rats.

• Authors: N Serbource-Goguel, D Porquet, J Feger, G Durand, M Appel, J Agneray
• Journal: Annals of nutrition & metabolism (Ann Nutr Metab) Vol. 29 Issue 2 Pg. 119-28 ( 1985) ISSN: 0250-6807 [Print] Switzerland
• Vernacular Title: Utilisation du fructose par l'hépatocyte isolé de rat nourri Zucker obèse fa/fa. I. Orientation métabolique du fructose en l'absence de modulateur.
• PMID: 4039549 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Lactates
• Liver Glycogen
• Pyruvates
• Fructose
• Glucose
• Glycerol

Topics

• Animals
• Fructose (metabolism)
• Glucose (analysis)
• Glycerol (analysis)
• Lactates (analysis)
• Liver (cytology, metabolism)
• Liver Glycogen (biosynthesis)
• Male
• Pyruvates (analysis)
• Rabbits
• Rats
• Rats, Inbred Strains (metabolism)
• Rats, Mutant Strains (metabolism)
• Rats, Zucker (metabolism)