Electrophoresis of muscle proteins is not a method for diagnosis of malignant hyperthermia susceptibility.

Abstract

The authors used denaturing polyacrylamide slab gel electrophoresis, employing 7.5-20% and 15-20% polyacrylamide gradients, to compare total skeletal muscle proteins of 12 normal and 19 malignant hyperthermia (MH)-susceptible individuals. The patients' MH status was determined by contracture testing. No consistent qualitative or quantitative differences could be detected. Because MH is believed to be triggered by a loss of control of sarcoplasmic [Ca2+], the authors compared: 1) the Ca2+-binding proteins of eight normal and ten MH muscles using Ca2+-dependent electrophoretic mobility shifts and a transblot/45CaCl2 overlay technique; and 2) the total protein composition of a heavy sarcoplasmic reticulum fraction isolated from five normal and nine MH muscles. Again, no differences were detected. Finally, a similar electrophoretic study was conducted to compare the total protein composition of five normal and six MH-susceptible, central core disease-affected human muscles. No significant differences could be observed. It appears, therefore, that simple electrophoretic techniques cannot be applied in the diagnosis of MH susceptibility.

• Authors: M P Walsh, A K Brownell, V Littmann, R T Paasuke
• Journal: Anesthesiology (Anesthesiology) Vol. 64 Issue 4 Pg. 473-9 (Apr 1986) ISSN: 0003-3022 [Print] United States
• PMID: 3963453 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Muscle Proteins
• Caffeine
• Halothane

Topics

• Caffeine (pharmacology)
• Electrophoresis, Polyacrylamide Gel
• Halothane (pharmacology)
• Humans
• Malignant Hyperthermia (diagnosis, metabolism, physiopathology)
• Molecular Weight
• Muscle Contraction (drug effects)
• Muscle Proteins (analysis)
• Sarcoplasmic Reticulum (analysis)