Abstract |
This study aimed to explore the potential roles of fractalkine/CX3CR1, primarily expressed in vascular endothelial cells and has recently been identified in dental pulp cells at sites of pulp tissue inflammation, not only in inflammation but also in pulp hard tissue formation. To this end, cultured human dental pulp cells were grown in 10% FBS-supplemented α-MEM. Fractalkine was introduced to the culture, and COX-2 and dentin sialophosphoprotein (DSPP) expression levels were evaluated via western blotting. Real-time PCR was used to examine BMP-2 and Osterix mRNA expression. Calcified nodule formation was evaluated with Alizarin red staining. Results revealed that fractalkine increased COX-2 protein expression, calcified nodule formation, and BMP-2 and Osterix mRNA expression in a concentration- and time-dependent manner. DSPP protein expression also increased upon fractalkine addition. This effect of fractalkine on expression of DSPP protein was inhibited in the presence of the CX3CR1 inhibiter ADZ8797. In conclusion, our findings suggest a dual role for fractalkine in promoting pulp inflammation via COX-2 production and contributing to pulp hard tissue formation by stimulating the expression of hard tissue formation markers.
|
Authors | Natsuko Gomyo-Furuya, Naoto Kamio, Takahiro Watanabe, Tomomi Hayama, Joji Fukai, Kosei Kuramochi, Kento Nakanishi, Arata Watanabe, Tatsu Okabe, Kiyoshi Matsushima |
Journal | Biomedical research (Tokyo, Japan)
(Biomed Res)
Vol. 44
Issue 6
Pg. 257-264
( 2023)
ISSN: 1880-313X [Electronic] Japan |
PMID | 38008424
(Publication Type: Journal Article)
|
Chemical References |
- Chemokine CX3CL1
- Cyclooxygenase 2
- Extracellular Matrix Proteins
- RNA, Messenger
- CX3CL1 protein, human
|
Topics |
- Humans
- Cell Differentiation
- Cells, Cultured
- Chemokine CX3CL1
(genetics, metabolism)
- Cyclooxygenase 2
(genetics, metabolism)
- Dental Pulp
- Endothelial Cells
- Extracellular Matrix Proteins
(metabolism)
- Inflammation
(metabolism)
- Odontoblasts
(metabolism)
- RNA, Messenger
(metabolism)
|