Notopterol Inhibits the NF-κB Pathway and Activates the PI3K/Akt/Nrf2 Pathway in Periodontal Tissue.

Abstract

Notopterol, an active component isolated from the traditional Chinese medicine Notopterygium incisum Ting ex H.T. Chang, exerts anti-inflammatory activity in rheumatoid arthritis. However, its roles in suppression of inflammatory insults and halting progression of tissue destruction in periodontitis remain elusive. In this study, we reveal that notopterol can inhibit osteoclastogenesis, thereby limiting alveolar bone loss in vivo. In vitro results demonstrated that notopterol administration inhibited synthesis of inflammatory mediators such as IL-1β, IL-32, and IL-8 in LPS-stimulated human gingival fibroblasts. Mechanistically, notopterol inhibits activation of the NF-κB signaling pathway, which is considered a prototypical proinflammatory signaling pathway. RNA sequencing data revealed that notopterol activates the PI3K/protein kinase B (Akt)/NF-E2-related factor 2 (Nrf2) signaling pathway in LPS-stimulated human gingival fibroblasts, a phenomenon validated via Western blot assay. Additionally, notopterol treatment suppressed reactive oxygen species levels by upregulating the expression of antioxidant genes, including heme oxygenase 1 (HO-1), NAD(P)H quinone oxidoreductase 1 (NQO1), catalase (CAT), and glutathione reductase (GSR), indicating that notopterol confers protection against oxidative stress. Notably, inhibition of Akt activity by the potent inhibitor, MK-2206, partially attenuated both anti-inflammatory and antioxidant effects of notopterol. Collectively, these results raise the possibility that notopterol relieves periodontal inflammation by suppressing and activating the NF-κB and PI3K/AKT/Nrf2 signaling pathways in periodontal tissue, respectively, suggesting its potential as an efficacious treatment therapy for periodontitis.

• Authors: Jianpeng Zhou, Peilei Shi, Rui Ma, Xudong Xie, Lei Zhao, Jun Wang
• Journal: Journal of immunology (Baltimore, Md. : 1950) (J Immunol) Vol. 211 Issue 10 Pg. 1516-1525 (11 15 2023) ISSN: 1550-6606 [Electronic] United States
• PMID: 37819772 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright © 2023 by The American Association of Immunologists, Inc.

Chemical References

• NF-kappa B
• Proto-Oncogene Proteins c-akt
• NF-E2-Related Factor 2
• Phosphatidylinositol 3-Kinases
• notopterol
• Lipopolysaccharides
• Anti-Inflammatory Agents
• Antioxidants
• Heme Oxygenase-1

Topics

• Humans
• NF-kappa B (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• NF-E2-Related Factor 2 (metabolism)
• Phosphatidylinositol 3-Kinases (metabolism)
• Lipopolysaccharides (pharmacology)
• Anti-Inflammatory Agents (pharmacology)
• Antioxidants
• Periodontitis
• Heme Oxygenase-1 (metabolism)