Abstract | Background: Objective: The aim of this study was to present the potential model to be developed as a urethral stricture. Methods: True experimental laboratory research was conducted by using Male New Zealand rabbits (Oryctolagus cuniculus), which were divided into 5 groups; control, placebo, and 3 treatment groups (TGF-β1 injection of 1 µg, 2 µg, 4 µg). Urethrography, histopathological analysis, and evaluation of total collagen formation of the urethral wall were performed after 6 weeks. Results: An increase in the dose of TGF-β1 decreased the mean rabbit's urethral lumen diameter (29.3% in the 2µg group and 34% in the 4µg group) compared to controls. Three rabbits decreased as much as ≤ 50% in urethral lumen diameter. Significant increases in total collagen density in the periluminal and peripheral urethral spongiosum were noted by increasing doses of TGF-β1. The percentage of urethral lumen diameter has a strong negative correlation with periluminal total collagen density (r = -0,798; p = 0,000) and very strong negative correlation with peripheral spongiosa total collagen density (r = -0,748, p = 0,000). Conclusion: TGF-β1 plays a role in changing total collagen compositions of the rabbit's urethral wall, decreasing the urethral lumen diameter. Further research with increasing doses of TGF-β1 is needed to determine the effective dose of TGF-β1 in inducing urethral stricture.
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Authors | Paksi Satyagraha, Hamid Hunaif Dhofi Alluza, I Made Udiyana Indradiputra, Athaya Febriantyo Purnomo, Pradana Nurhadi, Kenty Wantri Anita, Yuyun Yueniwati, Happy Kurnia Permatasari, Basuki Bambang Purnomo |
Journal | Medical archives (Sarajevo, Bosnia and Herzegovina)
(Med Arch)
Vol. 77
Issue 3
Pg. 189-193
( 2023)
ISSN: 1986-5961 [Electronic] Bosnia and Herzegovina |
PMID | 37700922
(Publication Type: Journal Article)
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Copyright | © 2023 Paksi Satyagraha, Hamid Hunaif Dhofi Alluza,I Made Udiyana Indradiputra, Athaya Febriantyo Purnomo, Pradana Nurhadi, Kenty Wantri Anita, Yuyun Yueniwati, Happy Kurnia Permatasari, Basuki Bambang Purnomo. |
Chemical References |
- Transforming Growth Factor beta1
- Collagen
- rimiterol
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Topics |
- Male
- Rabbits
- Animals
- Urethral Stricture
- Transforming Growth Factor beta1
- Urethra
- Collagen
- Models, Animal
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