Abstract | OBJECTIVES: METHODS: Cell-based assays (CBAs), immunoprecipitation-immunoblot assays, and various immunoblotting techniques were used in the study. RESULTS: We demonstrate that DM patient autoantibodies recognize MDA5 epitopes in a native conformation-dependent manner. Furthermore, we identified the central helicase domain formed by Hel1, Hel2i, Hel2, and pincer (3Hel) as the major epitopes. As proof of principle, the purified 3Hel efficiently absorbed MDA5 autoantibodies from patient sera through immunoprecipitation-immunoblot assay. CONCLUSION: Our study uncovers the nature of antigen epitopes on MDA5 and provides guidance for diagnosis and targeted therapeutic approach development.
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Authors | Yongxin Mo, Yan Ye, Lisheng Peng, Xiaobo Sun, Xiaofen Zhong, Rui Wu |
Journal | Rheumatology (Oxford, England)
(Rheumatology (Oxford))
(Aug 08 2023)
ISSN: 1462-0332 [Electronic] England |
PMID | 37551942
(Publication Type: Journal Article)
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Copyright | © The Author(s) 2023. Published by Oxford University Press on behalf of the British Society for Rheumatology. |