The amount of
copper ions in the environment has an immediate effect on ecology and food safety,
Menkes syndrome and
Wilson's disease cause accumulation and deficiency of
copper ions in the body, respectively, and
neurodegenerative diseases are also closely related to
copper ion levels. However, the current
copper ion detection technology has a high cost, complex operation, and other disadvantages. In this study, a ratiometric
fluorescent probe (RB-DH) was rationally constructed to detect
copper ions by coupling
benzothiazole to
rhodol derivatives. It can be used to determine
copper ion concentrations in water samples, agricultural products, cells, and zebrafish. Importantly, due to the reversible response of RB-DH to
copper ions, the fluctuation of intracellular
copper ion content during the release of
copper ion-related drugs (
Copper gluconate and
D-penicillamine) was successfully monitored with RB-DH for the first time. This study demonstrates RB-DH's potential application in the evaluation of related drug release effects and serves as a guide for the establishment of portable detection techniques for other important substances.