Abstract |
Waning vaccine-induced immunity, coupled with the emergence of SARS-CoV-2 variants, has inspired the widespread implementation of COVID-19 booster vaccinations. Here, we evaluated the potential of the GX-19N DNA vaccine as a heterologous booster to enhance the protective immune response to SARS-CoV-2 in mice primed with either an inactivated virus particle (VP) or an mRNA vaccine. We found that in the VP-primed condition, GX-19N enhanced the response of both vaccine-specific antibodies and cross-reactive T Cells to the SARS-CoV-2 variant of concern (VOC), compared to the homologous VP vaccine prime-boost. Under the mRNA-primed condition, GX-19N induced higher vaccine-induced T Cell responses but lower antibody responses than the homologous mRNA vaccine prime-boost. Furthermore, the heterologous GX-19N boost induced higher S-specific polyfunctional CD4+ and CD8+ T cell responses than the homologous VP or mRNA prime-boost vaccinations. Our results provide new insights into booster vaccination strategies for the management of novel COVID-19 variants.
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Authors | Yong Bok Seo, Ara Ko, Duckhyang Shin, Junyoung Kim, You Suk Suh, Juyoung Na, Ji In Ryu, Suyeon Lee, Min Ji Oh, Young Chul Sung |
Journal | International journal of molecular sciences
(Int J Mol Sci)
Vol. 24
Issue 11
(Jun 05 2023)
ISSN: 1422-0067 [Electronic] Switzerland |
PMID | 37298704
(Publication Type: Journal Article)
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Chemical References |
- Antibodies, Neutralizing
- Antibodies, Viral
- COVID-19 Vaccines
- DNA
- GX-19N vaccine
- mRNA Vaccine
- RNA, Messenger
- Vaccines, Inactivated
- Interferon-gamma
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Topics |
- Animals
- Humans
- Mice
- Antibodies, Neutralizing
- Antibodies, Viral
- COVID-19
(prevention & control)
- COVID-19 Vaccines
- DNA
- RNA, Messenger
(genetics)
- SARS-CoV-2
- Vaccination
- Vaccines, Inactivated
- Interferon-gamma
(immunology, metabolism)
- T-Lymphocytes
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