Cadmium exposure caused cardiotoxicity in common carps (Cyprinus carpio L.): miR-9-5p, oxidative stress, energetic impairment, mitochondrial division/fusion imbalance, inflammation, and autophagy.

Abstract

Cadmium (Cd), a toxic heavy metal pollutant, is a threat to human and eatable fish health. Common carps are widely cultivated and eaten by humans. However, there are no reports about Cd-damaged common carp hearts. Our experiment attempted to investigate the cardiotoxicity of Cd to common carps by establishing a common carp Cd exposure model. Our results showed that Cd injured hearts. Moreover, Cd treatment induced autophagy via miR-9-5p/Sirt1/mTOR/ULK1 pathway. Cd exposure caused oxidant/antioxidant imbalance and oxidative stress; and led to energetic impairment. Energetic impairment partook in oxidative stress-mediated autophagy through AMPK/mTOR/ULK1 pathway. Furthermore, Cd caused mitochondrial division/fusion imbalance and resulted in inflammatory injury via NF-κB-COX-2-PTGEs and NF-κB-COX-2-TNF-α pathways. Oxidative stress mediated mitochondrial division/fusion imbalance, further induced inflammation and autophagy via OPA1/NF-κB-COX-2-TNF-α-Beclin1 and OPA1/NF-κB-COX-2-TNF-α/P62 pathways under Cd treatment. Taken together, miR-9-5p, oxidative stress, energetic impairment, mitochondrial division/fusion imbalance, inflammation, and autophagy participated in the mechanism of Cd-cardiotoxicity to common carps. Our study revealed harmful effect of Cd on hearts, and provided new information for researches of environmental pollutant toxicity.

• Authors: Yuhao Liu, Xu Lin, Zhiyu Hao, Meijin Yu, You Tang, Xiaohua Teng, Wei Sun, Lu Kang
• Journal: Fish & shellfish immunology (Fish Shellfish Immunol) Vol. 138 Pg. 108853 (Jul 2023) ISSN: 1095-9947 [Electronic] England
• PMID: 37245677 (Publication Type: Journal Article)
• Copyright: Copyright © 2023 Elsevier Ltd. All rights reserved.

Chemical References

• Cadmium
• NF-kappa B
• Tumor Necrosis Factor-alpha
• Cyclooxygenase 2
• MicroRNAs
• TOR Serine-Threonine Kinases

Topics

• Humans
• Animals
• Carps (metabolism)
• Cadmium (toxicity)
• NF-kappa B (metabolism)
• Cardiotoxicity
• Tumor Necrosis Factor-alpha (metabolism)
• Cyclooxygenase 2
• Oxidative Stress
• MicroRNAs (metabolism)
• Inflammation (chemically induced, veterinary)
• TOR Serine-Threonine Kinases (metabolism)
• Autophagy